Review



mims microscope 50 l sims  (CAMECA Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    CAMECA Inc mims microscope 50 l sims
    A Study design. After 2 months of age, 15 N-labeled mice were kept on AL, 20% CR or HFD for 12 months. B Multi-isotope imaging mass spectroscopy <t>(MIMS)</t> representation technique. C Validation of 15 N incorporation by MIMS imaging and quantification of 15 N/ 14 N ratio in cortical neurons from a 15 N-labeled mouse after chase with 14 N diet for 12 months. Each dot represents a single neuronal nucleus ( n = 21 nucleus from 2 cortical layers of 1 mouse) D MIMS of pancreatic islets from AL, CR, or HFD mice ( n = 3 islets per animal, n = 1 mouse per diet group). E Single cell analysis of 15 N/ 14 N ratios in beta cells at day 0 (no chase) and after 12 months (mo) on AL, HFD, or CR diet. Each dot represents a single beta cell nucleus, pooled from 3 different islets of 1 mouse per diet group. Total of n = 54 AL 0mo, n = 217 AL 12mo, n = 114 HFD 12m0, and n = 194 CR 12mo beta cells were analyzed. The dotted horizontal pink and black lines represent the mean and the lowest 15 N/ 14 N levels found in cortical neurons shown in ( C ). F Estimation of beta cells that are estimated to be LLC or young after 12 months on AL, CR, or HFD. B , D scale bar = 10 microns. The p values for all significantly different comparison are shown. Statistical analysis was conducted using one-way ANOVA with Benjamini, Krieger, and Yekutieli’s post-hoc test ( B ). All data presented as mean ± 95% confidence intervals (C.l.). A was created with BioRender.com. and released under a Creative Commons Attribution-Non-Commercial NoDerivs 4.0 International license.
    Mims Microscope 50 L Sims, supplied by CAMECA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mims+microscope+50+l+sims/mims+microscope+50+l+sims/pmc11493975-520-8-13
    Average 90 stars, based on 1 article reviews
    mims microscope 50 l sims - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Calorie restriction increases insulin sensitivity to promote beta cell homeostasis and longevity in mice"

    Article Title: Calorie restriction increases insulin sensitivity to promote beta cell homeostasis and longevity in mice

    Journal: Nature Communications

    doi: 10.1038/s41467-024-53127-2

    A Study design. After 2 months of age, 15 N-labeled mice were kept on AL, 20% CR or HFD for 12 months. B Multi-isotope imaging mass spectroscopy (MIMS) representation technique. C Validation of 15 N incorporation by MIMS imaging and quantification of 15 N/ 14 N ratio in cortical neurons from a 15 N-labeled mouse after chase with 14 N diet for 12 months. Each dot represents a single neuronal nucleus ( n = 21 nucleus from 2 cortical layers of 1 mouse) D MIMS of pancreatic islets from AL, CR, or HFD mice ( n = 3 islets per animal, n = 1 mouse per diet group). E Single cell analysis of 15 N/ 14 N ratios in beta cells at day 0 (no chase) and after 12 months (mo) on AL, HFD, or CR diet. Each dot represents a single beta cell nucleus, pooled from 3 different islets of 1 mouse per diet group. Total of n = 54 AL 0mo, n = 217 AL 12mo, n = 114 HFD 12m0, and n = 194 CR 12mo beta cells were analyzed. The dotted horizontal pink and black lines represent the mean and the lowest 15 N/ 14 N levels found in cortical neurons shown in ( C ). F Estimation of beta cells that are estimated to be LLC or young after 12 months on AL, CR, or HFD. B , D scale bar = 10 microns. The p values for all significantly different comparison are shown. Statistical analysis was conducted using one-way ANOVA with Benjamini, Krieger, and Yekutieli’s post-hoc test ( B ). All data presented as mean ± 95% confidence intervals (C.l.). A was created with BioRender.com. and released under a Creative Commons Attribution-Non-Commercial NoDerivs 4.0 International license.
    Figure Legend Snippet: A Study design. After 2 months of age, 15 N-labeled mice were kept on AL, 20% CR or HFD for 12 months. B Multi-isotope imaging mass spectroscopy (MIMS) representation technique. C Validation of 15 N incorporation by MIMS imaging and quantification of 15 N/ 14 N ratio in cortical neurons from a 15 N-labeled mouse after chase with 14 N diet for 12 months. Each dot represents a single neuronal nucleus ( n = 21 nucleus from 2 cortical layers of 1 mouse) D MIMS of pancreatic islets from AL, CR, or HFD mice ( n = 3 islets per animal, n = 1 mouse per diet group). E Single cell analysis of 15 N/ 14 N ratios in beta cells at day 0 (no chase) and after 12 months (mo) on AL, HFD, or CR diet. Each dot represents a single beta cell nucleus, pooled from 3 different islets of 1 mouse per diet group. Total of n = 54 AL 0mo, n = 217 AL 12mo, n = 114 HFD 12m0, and n = 194 CR 12mo beta cells were analyzed. The dotted horizontal pink and black lines represent the mean and the lowest 15 N/ 14 N levels found in cortical neurons shown in ( C ). F Estimation of beta cells that are estimated to be LLC or young after 12 months on AL, CR, or HFD. B , D scale bar = 10 microns. The p values for all significantly different comparison are shown. Statistical analysis was conducted using one-way ANOVA with Benjamini, Krieger, and Yekutieli’s post-hoc test ( B ). All data presented as mean ± 95% confidence intervals (C.l.). A was created with BioRender.com. and released under a Creative Commons Attribution-Non-Commercial NoDerivs 4.0 International license.

    Techniques Used: Labeling, Imaging, Mass Spectrometry, Biomarker Discovery, Single-cell Analysis, Comparison

    Related Articles

    Microscopy:

    Article Title: Calorie restriction increases insulin sensitivity to promote beta cell homeostasis and longevity in mice
    Article Snippet: Here, epoxy-embedded pancreas blocks were scanned using XRM (X-radia Versa 510, Zeiss) with the source set at 80 kV and 7 W power using a 4X power objective (final pixel size ~ 2 microns depending on final geometric magnification). .. Next, the same samples were transferred to a MIMS microscope (50 L SIMS, Cameca, France) and acquisition of isotope maps ( 15 N, 14 N, 12 C, and 32 S) was achieved using a cesium (Cs − ) beam. .. Islets were identified based on their x-ray contrast and their 3D coordinates acquired using XRM to guide tissue and islet sectioning using a Leica ultramicrotome to trim the block and cut 80-nm-thick sections for imaging .



    Similar Products

    mims microscope 50 l sims  (CAMECA Inc)
    90
    CAMECA Inc mims microscope 50 l sims
    A Study design. After 2 months of age, 15 N-labeled mice were kept on AL, 20% CR or HFD for 12 months. B Multi-isotope imaging mass spectroscopy <t>(MIMS)</t> representation technique. C Validation of 15 N incorporation by MIMS imaging and quantification of 15 N/ 14 N ratio in cortical neurons from a 15 N-labeled mouse after chase with 14 N diet for 12 months. Each dot represents a single neuronal nucleus ( n = 21 nucleus from 2 cortical layers of 1 mouse) D MIMS of pancreatic islets from AL, CR, or HFD mice ( n = 3 islets per animal, n = 1 mouse per diet group). E Single cell analysis of 15 N/ 14 N ratios in beta cells at day 0 (no chase) and after 12 months (mo) on AL, HFD, or CR diet. Each dot represents a single beta cell nucleus, pooled from 3 different islets of 1 mouse per diet group. Total of n = 54 AL 0mo, n = 217 AL 12mo, n = 114 HFD 12m0, and n = 194 CR 12mo beta cells were analyzed. The dotted horizontal pink and black lines represent the mean and the lowest 15 N/ 14 N levels found in cortical neurons shown in ( C ). F Estimation of beta cells that are estimated to be LLC or young after 12 months on AL, CR, or HFD. B , D scale bar = 10 microns. The p values for all significantly different comparison are shown. Statistical analysis was conducted using one-way ANOVA with Benjamini, Krieger, and Yekutieli’s post-hoc test ( B ). All data presented as mean ± 95% confidence intervals (C.l.). A was created with BioRender.com. and released under a Creative Commons Attribution-Non-Commercial NoDerivs 4.0 International license.
    Mims Microscope 50 L Sims, supplied by CAMECA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mims+microscope+50+l+sims/mims+microscope+50+l+sims/pmc11493975-520-8-13
    Average 90 stars, based on 1 article reviews
    mims microscope 50 l sims - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A Study design. After 2 months of age, 15 N-labeled mice were kept on AL, 20% CR or HFD for 12 months. B Multi-isotope imaging mass spectroscopy (MIMS) representation technique. C Validation of 15 N incorporation by MIMS imaging and quantification of 15 N/ 14 N ratio in cortical neurons from a 15 N-labeled mouse after chase with 14 N diet for 12 months. Each dot represents a single neuronal nucleus ( n = 21 nucleus from 2 cortical layers of 1 mouse) D MIMS of pancreatic islets from AL, CR, or HFD mice ( n = 3 islets per animal, n = 1 mouse per diet group). E Single cell analysis of 15 N/ 14 N ratios in beta cells at day 0 (no chase) and after 12 months (mo) on AL, HFD, or CR diet. Each dot represents a single beta cell nucleus, pooled from 3 different islets of 1 mouse per diet group. Total of n = 54 AL 0mo, n = 217 AL 12mo, n = 114 HFD 12m0, and n = 194 CR 12mo beta cells were analyzed. The dotted horizontal pink and black lines represent the mean and the lowest 15 N/ 14 N levels found in cortical neurons shown in ( C ). F Estimation of beta cells that are estimated to be LLC or young after 12 months on AL, CR, or HFD. B , D scale bar = 10 microns. The p values for all significantly different comparison are shown. Statistical analysis was conducted using one-way ANOVA with Benjamini, Krieger, and Yekutieli’s post-hoc test ( B ). All data presented as mean ± 95% confidence intervals (C.l.). A was created with BioRender.com. and released under a Creative Commons Attribution-Non-Commercial NoDerivs 4.0 International license.

    Journal: Nature Communications

    Article Title: Calorie restriction increases insulin sensitivity to promote beta cell homeostasis and longevity in mice

    doi: 10.1038/s41467-024-53127-2

    Figure Lengend Snippet: A Study design. After 2 months of age, 15 N-labeled mice were kept on AL, 20% CR or HFD for 12 months. B Multi-isotope imaging mass spectroscopy (MIMS) representation technique. C Validation of 15 N incorporation by MIMS imaging and quantification of 15 N/ 14 N ratio in cortical neurons from a 15 N-labeled mouse after chase with 14 N diet for 12 months. Each dot represents a single neuronal nucleus ( n = 21 nucleus from 2 cortical layers of 1 mouse) D MIMS of pancreatic islets from AL, CR, or HFD mice ( n = 3 islets per animal, n = 1 mouse per diet group). E Single cell analysis of 15 N/ 14 N ratios in beta cells at day 0 (no chase) and after 12 months (mo) on AL, HFD, or CR diet. Each dot represents a single beta cell nucleus, pooled from 3 different islets of 1 mouse per diet group. Total of n = 54 AL 0mo, n = 217 AL 12mo, n = 114 HFD 12m0, and n = 194 CR 12mo beta cells were analyzed. The dotted horizontal pink and black lines represent the mean and the lowest 15 N/ 14 N levels found in cortical neurons shown in ( C ). F Estimation of beta cells that are estimated to be LLC or young after 12 months on AL, CR, or HFD. B , D scale bar = 10 microns. The p values for all significantly different comparison are shown. Statistical analysis was conducted using one-way ANOVA with Benjamini, Krieger, and Yekutieli’s post-hoc test ( B ). All data presented as mean ± 95% confidence intervals (C.l.). A was created with BioRender.com. and released under a Creative Commons Attribution-Non-Commercial NoDerivs 4.0 International license.

    Article Snippet: Next, the same samples were transferred to a MIMS microscope (50 L SIMS, Cameca, France) and acquisition of isotope maps ( 15 N, 14 N, 12 C, and 32 S) was achieved using a cesium (Cs − ) beam.

    Techniques: Labeling, Imaging, Mass Spectrometry, Biomarker Discovery, Single-cell Analysis, Comparison